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Lymph node, cerebrospinal fluid (CSF), pleural fluid, respiratory secretions, sputum, ear notches. Please note that processing of ear notch samples may delay the sample turnaround time.

This assay targets the DNA of a portion of the rpoB, pag, and cap genes from the bacterium Bacillus anthracis. Positive samples for the rpoB gene will subsequently be tested for the presence of the pag and cap genes located on the pX01 and pX02 plasmids, respectively. Research has shown that pathogenic B. anthracis requires the presence of both the pX01 and pX02 plasmids. Apathogenic strains, such as those used for vaccines, may only have one plasmid. Also, other bacilli have tested positive for rpoB and one of the plasmids.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

Samples must be received by 11:00 am of the test day along with prior notification of the laboratory to ensure the reported turn around time.

The submitter is responsible for adherence to sample shipping regulations. Suggested Shipping Protocol: Place primary sample container (blood collection tube) inside a secondary leak proof container such as a sealable bag with sufficient absorbent material (e.g. paper towels) to absorb liquid contents in the event of a spill or breakage; ship on frozen ice pack. Place paperwork inside separate sealable bag. Place all items above within an insulated inner box or pouch and label inner package “Anthrax suspect”. Do not write this on the outer box. Place the inner package inside a rigid outer cardboard box. Label for Biological Substance Category B, UN3373. Next day delivery is recommended. Review our submission guide for packaging and shipping information.

Do not place any other samples in the box. If the sample is positive, all other samples in the same container will be destroyed.

Necropsy should not be performed if anthrax is suspected. Peripheral blood is the preferred specimen in cattle. Send blood samples in red top tube without serum separator gel (plastic tube is preferred) or EDTA. Do not send syringes with needles still attached. This practice is dangerous and inappropriate. Samples submitted with needles will be rejected for testing.

Please call [(701) 231-7527 or (701) 231-8307] or email (using our contact form) prior to shipping or dropping off your specimen(s). Failure to notify the laboratory prior to submitting the specimen may result in a delay in test results.

In addition to blood, submit exudate or affected lymph nodes for horses and swine as blood may be falsely negative.

Slides

Histologic evaluation of biopsy specimen from a skin mass or partial organ. Price includes routine evaluation, description and diagnosis of 1 site or tissue. This includes lung, bone, GI, and multiple skin punch or wedge biopsies.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

We recommend the use of tissue ink to denote surgical margin when submitting tumors. See our guide for using tissue ink to denote surgical margins. Please use the Biopsy/Surgical Pathology Submission Form for all surgical pathology submissions. For dermatopathology submissions, please use our Dermatopathology Submission Form.

Immunohistochemistry is available for an additional fee. See test catalog for available cell markers and agents.

Slides

Histologic evaluation of biopsy specimen from a whole organ or organ system. Price includes routine evaluation, description, and diagnosis of 1 site or tissue. Examples include spleen, uterus and ovaries, testes, and mammary chain.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

We recommend the use of tissue ink to denote surgical margin when submitting tumors. See our guide for using tissue ink to denote surgical margins. Please use the Biopsy/Surgical Pathology Submission Form for all surgical pathology submissions.

Immunohistochemistry is available for an additional fee. See test catalog for available cell markers and agents.

Performed by GC/MS/MS.

Equine | Ruminants

Ship overnight on cold packs. Urine should be submitted in a leakproof container.

*Centrifuge blood collected in anticoagulant (EDTA or heparin); remove plasma from cells and submit plasma in a separate tube.

Collect samples from acutely affected animals. Treated animals will have reduced to non-detectable amounts of toxin.

Isolation and identification of bacteria from blood. Sample must be submitted in blood culture bottle. An additional fee is charged for antibiotic susceptibilities.

Bovine | Canine | Feline | Equine | Caprine | Porcine | Wildlife Exotic | Other

For best results, ship samples overnight (if possible) at room temperature in a leak proof container.

Aseptic collection in an appropriate culture bottle are critical for recovery of organisms. Please visit our Microbiology page for blood culture collection instructions. Contact the laboratory with any questions.

Bone marrow (humerus), about 30 grams

Estimate of residual fat in bone marrow using an air dry technique.

Bovine | Equine | Ovine | Caprine | Wildlife Exotic

Place sample in a zip lock bag, seal well, keep refrigerated and send on ice-pack. Prevent damage during transit by placing in a solid container.

Typically, normal large animals have more than 80% fat in bone marrow and animals suspected of death by starvation have less than 20% residual fat. The assay provides support to observations made by veterinarians and pathologists of fat atrophy in organs and death by starvation.

If animals die in the winter and freeze, intact femur bone can provide adequate bone marrow for analysis for months after death as long as carcasses are frozen. If veterinarians have difficulty removing bone marrow from the femur, the entire femur can be submitted to the lab and the lab will remove the bone marrow, please call the lab in this case.

Please submit about 30 grams of bone marrow from the medullary cavity (no medullary bone). Young animals may have a smaller sample volume. If necessary, sample both femurs to acquire enough bone marrow.

Placenta

This assay targets the DNA of a portion of the glycoprotein B gene from Bovine herpesvirus-1 and RNA of the 5’UTR segment of Bovine viral diarrhea virus. Please be aware that this assay could detect vaccine strains if vaccination occurs within a few days or weeks of testing.

Bovine

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Leptospira PCR can be requested for an additional fee.

Detection of bovine coronavirus by fluorescent antibody (FA) testing.

Does not react with bovine adenovirus types 1, 3, and 5, bovine leukemia virus, bovine parvovirus, bovine respiratory syncytial virus, bluetongue virus, bovine viral diarrhea virus, bovine herpesvirus-1 (IBR), parainfluenza virus-3, reovirus, rabies recombinant nucleoprotein (rNP), or vesicular stomatitis virus Indiana and New Jersey strains

Bovine

Slides (charged/adhesive)

Detection of bovine coronavirus by immunohistochemistry using anti-bovine coronavirus monoclonal antibody (clone BC6-4).

Bovine

In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

This assay targets the RNA of a portion of the N gene from Bovine coronavirus (BCoV), RNA of a portion of the VP6 gene from Bovine Rotavirus group A (BRV), and DNA of a portion of the Cryptosporidium oocysts wall protein gene (COWP) from Cryptosporidium parvum (Crypto).

Bovine

To ensure inclusion in the test run, samples should arrive the day prior to test day.

The neonatal diarrhea study panel should be requested for young calves. The neonatal diarrhea study includes bovine diarrhea PCR, bacterial culture, and detection of E. coli virulence factors by PCR if the animal is less than two weeks of age.

Due to the likelihood of positive samples and lack of validation data, the laboratory will not pool samples.

Detection of antibody to bovine leukemia virus by enzyme-linked immunosorbent assay (ELISA).

Bovine

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

APHIS-approved lab for Import/Export. Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, they will be tested the following scheduled test day. STAT testing may be available, contact the lab for more information.

Colostrum or milk

This targets the DNA of a portion of the pol gene from Bovine leukemia virus.

Bovine

To ensure inclusion in the test run, samples should arrive the day prior to test day.

This targets the DNA of a portion of the pol gene from Bovine leukemia virus.

Bovine

To ensure inclusion in the test run, samples should arrive the day prior to test day. We recommend notifying the Laboratory prior to submitting large cases (>100 individuals) and to submit early in the week to allow for ample processing time.

Package samples appropriately based on weather conditions. Frozen EDTA whole blood samples cannot be tested.

Samples will be pooled by the laboratory in sequential order as listed on the Submission Form/Sample Identification Form. Broken or missing samples will not be shifted or replaced with additional samples. However, if a retest is required, you will only be charged for the actual number of retested individuals. Samples will be pooled by the laboratory in groups of 5. Retest of positive pools is $25.00 per individual sample (5 individuals per pool X $25 = $125 per retested pool).

This test is helpful to detect BLV before animals seroconvert, but not recommended as a replacement for the serology test.

Detection of bovine parainfluenza type-3 by fluorescent antibody (FA) testing.

Bovine

Nasal or oropharyngeal swabs

This assay targets the DNA of a portion of the glycoprotein B gene of Bovine herpesvirus-1, RNA of a portion of the N gene from the Bovine coronoavirus, RNA of the 5’UTR segment of Bovine viral diarrhea virus, and RNA of the F gene from the Bovine respiratory syncytial virus. Please be aware that this assay could detect vaccine strains if vaccination occurs within a few days or weeks of testing.

Bovine

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Submit swabs in sterile, redtop tubes (without additives) or other sterile screwcap containers to with a small amount of saline (enough to cover the fibrous head of the swab) to maintain moisture. Viral transport media and universal transport media are also acceptable. Gel, such as culturette media or the type found in serum separator tubes, can inhibit PCR and should not be tested. Bacterial transport containers are not appropriate for PCR testing. If bacterial culture is also requested, please submit a second swab in the appropriate bacterial transport container.

Detection of bovine respiratory syncytial virus by fluorescent antibody (FA) testing.

Bovine

Slides (charged/adhesive)

Detection of bovine respiratory syncytial virus by immunohistochemistry using anti-BRSV polyclonal antibodies.

Bovine

In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

Detection of bovine viral diarrhea virus 1 and 2 by fluorescent antibody (FA) testing.

Bovine | Wildlife Exotic

Detects both type I and II BVDV.

Slides (charged/adhesive)

Detection of Bovine viral diarrhea virus by immunohistochemistry using anti-BVDV E0 monoclonal antibody. Initial charge covers up to nine ear notches. Each additional ear notch is $5.00.

Bovine | Other

In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

See submission guide for additional information.

Serum, milk, lung, kidney, intestine, heart, lymph node, placenta

This assay targets the RNA of the 5’UTR segment of the Bovine viral diarrhea virus. This assay can be tested with BoHV-1 (See Bovine Abortion Multiplex) or with BoHV-1, BCoV and BRSV (See Bovine Respiratory Disease Multiplex).

Bovine | Ovine | Caprine | Wildlife Exotic | Other

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Our BVD PCR assays do not differentiate between persistent/chronic infection (PI) and transient/acute infection (TI). See BVDV submission guide for sample collection, submission, and result interpretation information. Ear notches must be submitted in sterile container (red top tube without preservatives or gel, screw top container). Please DO NOT submit ear notches in Ziploc bags or WhirlPaks as processing may be significantly delayed. Please note: We do not currently accept AllFlex Tissue Sampling devices.

EN, EDTA whole blood, or serum samples can be tested in pools (See Bovine Viral Diarrhea Virus (pooled) PCR).

Serum

This assay targets the RNA of the 5’UTR segment of the Bovine viral diarrhea virus. Please be aware that this assay could detect vaccine strains if vaccination occurs within a few days or weeks of testing.

Bovine | Other

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Package samples appropriately for the weather conditions. Frozen EDTA whole blood samples cannot be tested; however, frozen serum is acceptable. If freezing is a concern due to weather conditions, please submit serum.

Our BVD PCR assays do not differentiate between persistent/chronic infection (PI) and transient/acute infection (TI). See BVDV submission guide for sample collection, submission, and result interpretation information. Please note: We do not currently accept AllFlex Tissue Sampling devices.

Ear notches must be submitted in sterile container (red top tube without gel or preservatives, screw top container). Please do not submit ear notches in Ziploc bags or Whirl-paks as processing may be significantly delayed.

Large numbers of samples (>50) should be submitted the day before scheduled test day to ensure reported turn around time.

Pools will be made at the lab in groups of up to 15 samples. Samples will be pooled by the laboratory in sequential order as listed on the Submission Form/Sample Identification Form. Broken or missing samples will not be shifted or replaced with additional samples. However, if a retest is required, you will only be charged for the actual number of retested individuals.

Retest of positive individual samples is $35.00 per/sample.

Perirenal fat (20 g)

Liver (20 g)

Detection of desmethyl bromethalin, a biological metabolite of bromethalin by LC-MS/MS.

Any

Submit on ice packs or frozen.

Detection of antibody to Brucella abortus by buffered acidified plate antigen (BAPA) testing.

Bovine | Bison | Equine | Porcine | Elk | Moose |Deer | Other

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

VS form 4-33 Brucellosis Test Record or NDSU Serology Form suggested. Official Identification recommended on sample and submission form with veterinarian signature and license number. APHIS-approved lab for Import/Export. The type of Brucella test required is destination dependent. Submitter is responsible for checking destination requirements.

Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, the sample will be tested on the following scheduled test day. STAT testing may be available, contact the lab for more information.

Detection of antibody to Brucella abortus/suis by card agglutination testing.

3% card: Caprine | Ovine | Big Horn Sheep | Mountain Goat

8% card: Porcine (suis) | Canine (abortus) | Other

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

VS form 4-33 Brucellosis Test Record or NDSU Serology Form suggested. Official Identification recommended on sample and submission form with veterinarian signature and license number. APHIS-approved lab for Import/Export. The type of Brucella test required is destination dependent. Submitter is responsible for checking destination requirements.

Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, the sample will be included in the run on the following scheduled test day. STAT testing may be available, contact the lab for more information.

Detection of antibody to Brucella ovis by enzyme-linked immunosorbent assay (ELISA).

Ovine

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

APHIS-approved laboratory for Import/Export. Tests for antibody to Brucella ovis.

Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, the sample will be tested on the following scheduled test day. STAT testing may be available, contact the lab for more information.