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A $10.00 accession fee will be applied to all cases except EIAs.
Live animals and human samples are not accepted. Only fixed, non-human primate samples will be accepted.

Complete terms, conditions and fees

Test	Preferred Sample	Fee
Johne’s Disease (ELISA)	Serum or milk (1 ml minimum)	$ 7
Schedule	Days Tested WED \| FRI – OCT 1 TO MAY 31 WED – JUNE 1 TO SEPT 30 Turnaround Time 1-2 days
Description	Detection of antibody to Mycobacterium avium subsp. paratuberculosis by enzyme-linked immunosorbent assay (ELISA).
Species	Bovine \| Caprine \| Ovine
Shipping Requirements	To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.
Additional Instructions	APHIS-approved lab for Import/Export. Results are reported as S/P ratio (Sample/Positive) not a titer. This test is not currently approved by USDA for Ovine testing and results are reported with a disclaimer. Contact the laboratory prior to submitting large numbers of samples (>100 samples) to ensure sufficient reagents are available. Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, they will be run the following scheduled test day. STAT testing may be available, contact the lab for more information.

Johne’s Disease (individual) (PCR)	Feces (2 gram minimum), intestinal contents, intestine	$ 35
Schedule	Days Tested TUE Turnaround Time 1-3 days for individual samples
Description	This assay targets the DNA of a portion of the genome of the bacterium Mycobacterium avium subsp. paratuberculosis (aka: MAP, Johne’s).
Species	Bovine \| Ovine \| Caprine \| Wildlife Exotic \| Other
Shipping Requirements	To ensure inclusion in the test run, samples should arrive the day prior to test day.
Additional Instructions	See submission guide for sample collection and submission guidelines. Indicate whether the samples should be pooled or tested individually, otherwise submissions containing greater than 5 samples will be pooled. Fecal samples can be tested in pools of up to 5 samples. Pooling will be done at the laboratory. See Johne’s Disease (pooled) (PCR). The NDSU-VDL is an APHIS-approved laboratory to conduct official Johne’s testing. Approval is based on satisfactory annual proficiency test scores.

Johne’s Disease (pooled) (PCR)	Feces (2 gram minimum). Samples MUST be labeled with the sample number (1, 2, 3, etc.) that corresponds with the identification form. An additional charge will be added to cases if sample numbers are not included on the fecal sample.	$ 50
Schedule	Days Tested TUE Turnaround Time 2-15 days, depending on results, number of samples submitted, and time of year. Improperly labeled cases (i.e. those with only animal IDs on specimens), may be placed at the back of the queue and take up to 4 weeks for testing.
Description	This assay targets the DNA of a portion of the genome of the bacterium Mycobacterium avium subsp. paratuberculosis (aka: MAP, Johne’s).
Species	Bovine \| Ovine \| Caprine \| Wildlife Exotic \| Other
Additional Instructions	Samples are pooled at the laboratory in pools of up to 5 samples. Pooled testing can only be performed on fecal samples. Indicate whether the samples should be pooled or tested individually, otherwise submissions containing greater than 5 samples will be pooled. Retest of positive pools is $35.00 per individual sample (5 individuals per pool X $35 = $175 per retested pool). See our submission guide for sample collection and submission guidelines. View our example page to see pictures of how to label your samples. Results of pool cases may take up to 15 days, dependent on retesting of pools and time of year. Individual samples requested on pool cases will not be reported out before the pool retests are finalized. The NDSU-VDL is an APHIS-approved laboratory to conduct official Johne’s testing. Approval is based on satisfactory annual proficiency test scores.