Johne’s Disease (ELISA)

Preferred Sample

Serum or milk (1 ml minimum)




Days Tested


Turnaround Time

1-2 days


Detection of antibody to Mycobacterium avium subsp. paratuberculosis by enzyme-linked immunosorbent assay (ELISA).


Bovine | Caprine | Ovine


Shipping Requirements

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

Additional Instructions

APHIS-approved lab for Import/Export. Results are reported as S/P ratio (Sample/Positive) not a titer. This test is not currently approved by USDA for Ovine testing and results are reported with a disclaimer.

Contact the laboratory prior to submitting large numbers of samples (>100 samples) to ensure sufficient reagents are available.

Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, they will be run the following scheduled test day. STAT testing may be available, contact the lab for more information.