Bacillus anthracis (Anthrax) (PCR)

Preferred Sample

Whole blood, spleen

Fee

$60

Acceptable Samples

Lymph node, cerebrospinal fluid (CSF), pleural fluid, respiratory secretions, sputum, ear notches. Please note that processing of ear notch samples may delay the sample turnaround time.

Schedule

Days Tested

As needed

Turnaround Time

24 hours with prior notice

Description

This assay targets the DNA of a portion of the rpoB, pag, and cap genes from the bacterium Bacillus anthracis. Positive samples for the rpoB gene will subsequently be tested for the presence of the pag and cap genes located on the pX01 and pX02 plasmids, respectively. Research has shown that pathogenic B. anthracis requires the presence of both the pX01 and pX02 plasmids. Apathogenic strains, such as those used for vaccines, may only have one plasmid. Also, other bacilli have tested positive for rpoB and one of the plasmids.

Species

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

Shipping Requirements

Samples must be received by 11:00 am of the test day to ensure the reported turn around time.

Suggested Shipping Protocol: Place primary sample container (blood collection tube) inside a secondary leak proof container such as a sealable bag with sufficient absorbent material (e.g. paper towels) to absorb liquid contents in the event of a spill or breakage; ship on frozen ice pack. Place paperwork inside separate sealable bag. Place all items above within an insulated inner box or pouch and label inner package “Anthrax suspect”. Do not write this on the outer box. Place the inner package inside a rigid outer cardboard box. Label for Biological Substance Category B, UN3373. Next day delivery is recommended. See submission guide for packaging and shipping information.

Additional Instructions

Necropsy should not be performed if anthrax is suspected. Peripheral blood is the preferred specimen in cattle. Send blood samples in red top tube without serum separator gel (plastic tube is preferred) or EDTA. Do not send syringes with needles still attached. Please call prior to shipping or dropping off your specimen(s) (701) 231-7527 or (701) 231-8307. Failure to notify the laboratory prior to submitting the specimen may result in a delay in test results.

In addition to blood, submit exudate or affected lymph nodes for horses and swine as blood may be falsely negative.