Tritrichomonas foetus (Individual) (PCR)

Preferred Sample

Preputial Wash (2 ml minimum)

Fee

$25

Acceptable Samples

Fetus, intestine, lung, endometrium, cervico-vaginal Mucous

Schedule

Days Tested

TUE | THU

Turnaround Time

Next business day

Description

This targets the DNA of a portion of the 5.8S rRNA gene and interal transcribed spacer-1 (ITS-1) region of the protozoan parasite Tritrichomonas foetus.

Note: Swabs are not an acceptable sample type.

 

Species

Bovine

Shipping Requirements

To ensure inclusion in the test run, samples must be received the day prior to test day.

Send on cold pack/ice in sterile leak-proof container. Plastic tubes are preferred. If blood tubes are used, must be red top without serum separator gel or anticoagulant. Please do not send samples in Whirl-Paks as samples tend to leak and may lead to contamination of other samples.

It is the responsibility of the submitter to provide on the submission form the collection date, animal IDs, and indicate pooled or individual testing. Additionally, the best attempt should be made to get samples to the NDSU-VDL (arrive during business hours) within 5 days of collection. Samples >5 days old are suboptimal for testing and may result in false negative results. If submission information is missing or samples are >5 days old, the NDSU-VDL will test at its discretion and a comment will be added to the report.

Additional Instructions

An appropriately collected washing should be light pink and cloudy, with visible material and little blood. Clear samples and samples with significant blood or fecal contamination are not appropriate for testing and may be either rejected or have a disclaimer placed on test results.

See submission guide for sample collection and submission guidelines. It is important to select “Individual” or “Pool (up to 5 in pool)” on the submission sheet. The submitter is responsible for checking destination state requirements concerning pooling of samples for Tritrichomonas testing.