Lyme Disease- Borrelia burgdorferi (PCR)

Preferred Sample

EDTA whole blood (1 ml minimum), skin biopsy at site of lesion



Acceptable Samples

Tissues (lymph node, synovium), Fluid (cerebrospinal fluid, synovial), Culture in BSK-H, ticks*



Days Tested


Turnaround Time

1-3 business days from test day


This assay targets the DNA of a portion of the flagellin gene from multiple isolates of the spirochete bacteria Borrelia burgdorferi complex, including foreign genospecies. Samples or ticks are only accepted from veterinary species. Samples or ticks from humans and non-human primates are NOT accepted.


Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

Shipping Requirements

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Additional Instructions

The spleen or kidney should not be tested.

*Ticks must be identified as a vector species prior to testing, which include the blacklegged tick (deer tick) or the western blacklegged tick. The American dog tick/wood tick is not considered a vector species. Contact the NDSU Plant Diagnostic Lab or Extension Entomology for assistance with insect identification. We highly recommend blood from the patient be submitted over deer ticks. A negative or positive result from a deer tick does not necessarily mean the patient will have the same result.