Leptospira species (PCR)

Preferred Sample

Urine (1 mL for small animals; 30-50 mL for bovine submissions), EDTA whole blood (1 ml minimum), kidney, liver. Testing of urine and EDTA whole blood is recommended.

Fee

$35*

Schedule

Days Tested

MON

Turnaround Time

1-2 business days from test day

Description

This assay targets the DNA of a portion of the lipL32 gene from Leptospira bacterial species. Pathogenic species detectable include various serovars of L. alexanderi, L. borgpeterseni, L. interrogans, L. kirschneri, L. noguchii, L. santarosai, and L. weilii. This assay can be multiplexed with BoHV-1 and BVDV as part of the Bovine Abortion Multiplex.

*$35 for the first sample, $15 for an additional sample on the same animal.

Species

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

Shipping Requirements

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Samples must be submitted to the lab ASAP. Shipped cold (not frozen) and within 24 hours of collection and prior to antibiotic treatment to minimize the chance of false negative results. Studies suggest Leptospira degrade relatively rapidly in acidic urine; therefore, urine samples submitted more than 5 days after collection will not be tested as there is a high probability of false negative results.

Additional Instructions

Depending on the infection phase and nature of the disease, Leptospira may be detected in the blood and not urine or vice versa. A negative urine does not definitively prove the animal is negative for Leptospira as it can be intermittently shed in urine.