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The general investigation submission fee covers a routine laboratory study which may include the following tests: histopathology with special stains, bacteriology (2 aerobic cultures and 1 anaerobic), PCR (1 individual or 1 multiplex test), virology (2 FAs, 1 EM), Cryptosporidia, and fecal flotation.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

If submitting tissues for general investigation, send both fixed and fresh tissues. Delays in tissue fixation can significantly affect results. Ratio of tissue to 10% formalin should be approximately 1:10 to avoid under-fixation. In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

The general investigation submission fee covers a routine laboratory study for a whole animal or tissue samples submitted from a field necropsy.

For bovine fetus necropsy submission guidelines, please see our Novel Pneumonia Syndrome Fact Sheet.

*The general investigation submission fee covers a routine laboratory study which may include the following tests: histopathology with special stains, bacteriology (2 aerobic cultures and 1 anaerobic), PCR (1 individual or 1 multiplex test), virology (2 FAs, 1 EM), Cryptosporidia, and fecal flotation. Additional fees will apply for antibiotic sensitivities, immunohistochemistry, clinical pathology, toxicology, serology, referral laboratory charges, and additional PCR, virology, bacteriology, and parasitology tests. Additional animals on the same submission (from a common disease outbreak) will be charged at 1/2 the price.

Contact the laboratory for instructions prior to submitting cases involving insurance claims or litigation. Legal cases may be charged a $250.00 base price to cover the cost associated with chain of custody handling.

Screen includes aerobic culture with two sensitivities, transmission electron microscopy for rotavirus and coronavirus, and modified acid-fast stain for Cryptosporidium. If animal is less than two weeks of age, E. coli virulence genes by PCR are included.

Bovine only: The calf diarrhea PCR panel for rotavirus, coronavirus, and Cryptosporidium will be performed in lieu of electron microscopy and microscope evaluation of acid-fast stained smears.

*$60 for first sample, $50 for each additional sample

Bovine | Ovine | Caprine

Please note that these test methods are validated on individual samples only. The laboratory will not pool the samples due to potential false negative results.

Amniotic fluid, semen, lung, spleen

This assay targets the DNA of a portion of the pNC-5 gene from the apicomplexan parasite Neospora caninum.

Bovine | Canine | Equine | Ovine | Caprine | Wildlife Exotic |Other

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Detection of antibody to Neospora caninum by competitive enzyme-linked immunosorbent assay (c-ELISA).

Bovine

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, the sample will be tested on the following scheduled test day. STAT testing may be available, contact the lab for more information.

This is a NAHLN approved assay which screens for the matrix gene, allowing for subsequent follow-up testing for the virulent fusion gene. Approval to conduct surveillance testing is based upon satisfactory completion of the yearly proficiency test.

Avian | Wildlife Exotic

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Surveillance sample turn around time is 2 days from the day of submission.

Sample types vary. Refer to Avian Sample Collection for Influenza A and Newcastle Disease for complete sampling information.

Quantitation of nitrates by colorimetric methods and ion selective electrode.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other