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The general investigation submission fee covers a routine laboratory study which may include the following tests: histopathology with special stains, bacteriology (2 aerobic cultures and 1 anaerobic), PCR (1 individual or 1 multiplex test), virology (2 FAs, 1 EM), Cryptosporidia, and fecal flotation. Additional fees will apply for antimicrobial susceptibility testing, immunohistochemistry, clinical pathology, toxicology, serology, referral laboratory charges, and additional PCR, virology, bacteriology, and parasitology tests. Additional animals on the same submission (from a common disease outbreak) will be charged at 1/2 the price. For bovine fetus necropsy submission guidelines, please see our Novel Pneumonia Syndrome Fact Sheet.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

If submitting tissues for general investigation, send both fixed and fresh tissues. Delays in tissue fixation can significantly affect results. Ratio of tissue to 10% formalin should be approximately 1:10 to avoid under-fixation. In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.

The general investigation submission fee covers a routine laboratory study for a whole animal or tissue samples submitted from a field necropsy.

¹ Description of fees:

² Partial ashes returned.

For complete descriptions and breakdowns of fees, click here.

Contact the laboratory for instructions prior to submitting cases involving insurance claims or litigation. Legal cases may be charged a $250.00 base price to cover the cost associated with chain of custody handling.

Large animals arriving on enclosed trailers require removal of trailer stall dividers. Stall divider removal must be performed by the client. If the stall divider cannot be removed, then the large animal will not be unloaded.

Screen includes aerobic culture, transmission electron microscopy for rotavirus and coronavirus, and modified acid-fast stain for Cryptosporidium. If animal is less than two weeks of age, E. coli virulence genes by PCR are included.

Bovine only: The calf diarrhea PCR panel for rotavirus, coronavirus, and Cryptosporidium will be performed in lieu of electron microscopy and microscope evaluation of acid-fast stained smears.

Bovine | Ovine | Caprine

Please note that these test methods are validated on individual samples only. The laboratory will not pool the samples due to potential false negative results.

Amniotic fluid, lung, spleen

This assay targets the DNA of a portion of the pNC-5 gene from the apicomplexan parasite Neospora caninum.

Bovine | Canine | Equine | Ovine | Caprine | Wildlife Exotic |Other

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Detection of antibody to Neospora caninum by competitive enzyme-linked immunosorbent assay (c-ELISA).

Bovine

To avoid hemolysis, serum should be removed from the clot and aliquoted to another tube prior to shipping. Hemolysis affects serologic testing and may cause a sample to be rejected. Samples should be kept cold during transport. Avoid freeze-thaw cycles.

Note: Samples for serologic testing must be received during business hours the day before scheduled testing to ensure inclusion in the test run. If not, the sample will be tested on the following scheduled test day. STAT testing may be available, contact the lab for more information.

This is a NAHLN approved assay which screens for the matrix gene, allowing for subsequent follow-up testing for the virulent fusion gene. Approval to conduct surveillance testing is based upon satisfactory completion of the yearly proficiency test.

Avian | Wildlife Exotic

To ensure inclusion in the test run, samples should arrive the day prior to test day.

Surveillance sample turn around time is 2 days from the day of submission.

Sample types vary. Refer to Avian Sample Collection for Influenza A and Newcastle Disease for complete sampling information.

Sample is dried, ground, and extracted into an aluminum sulfate solution and quantified using an ion specific electrode.

Review the Proper Sampling for Nitrate in Feedstuffs guide for current collection and handling practices.

Quantitation of nitrates by colorimetric method. The half-life of nitrates in beef cattle exposed to nitrates is between 7.7 to 9 hours. Therefore, it would be at least five biologic half-lives (24–36 hours) before increased nitrate concentrations from excessive nitrate exposure diminish to normally expected values, which means that affected cattle should be tested preferably within 24 hours after a significant nitrate exposure. If the animal has been dead for more tan 24-36 hours (less time in hot weather), autolysis and bacterial contamination can case false positive results.

Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other

As soon as possible, remove serum from blood tube and store at refrigeration or preferably freezer temperature and ship to the lab on ice packs.

If possible, remove clear ocular fluid from globe and keep at refrigeration or freezer temperature prior to shipping and ship on ice pack.  If globe submitted, keep at refrigeration temperature and ship on ice packs as soon as possible to the lab.

See our Resources page for proper sampling instructions for nitrate in feedstuffs.

Nitrate and nitrite are derivatized and analyzed by GC/MS.

Any

Fluids submitted in preservative free test tubes. Serum must be removed from the clot after centrifugation. Ocular fluid should be removed from the eye globe and then frozen or submitted on ice, overnight whenever possible.

This test is appropriate for use when samples are pigmented and not appropriate for colorimetric analysis or when a quantified result is needed. If the animal has been dead for more tan 24-36 hours (less time in hot weather), autolysis and bacterial contamination can case false positive results.