Acceptable Samples |
Swabs
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Isolation and identification of Campylobacter species from diagnostic samples.
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Species |
Bovine | Canine | Feline | Equine | Caprine | Porcine | Wildlife Exotic | Other
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Shipping Requirements |
Send intestinal or fecal samples on ice pack overnight in a leak proof container. Fluid samples from reproductive sites must be submitted in a specific transport media and arrive at the lab no longer than 24-hours after collection.
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Additional Instructions |
Modified Cary-Blair or other fecal transport media may be used for fecal submissions.
For isolation of Campylobacter from reproductive sites, collection and proper transport is essential for isolation by culture. Weybridge transport media is recommended and is available for purchase from the Wisconsin Veterinary Diagnostic Laboratory Madison, WI. Telephone 608-262-5432. Ask for the media specialist. Allow sufficient time (3-5 working days) for delivery. Refer to abortion submission recommendations if Campylobacter is suspected.
Antimicrobial susceptibility testing is not performed on Campylobacter species at NDSU-VDL.
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the DNA of a portion of the ISCfe-1 gene from the bacterium C. fetus subsp. venerealis, the agent of bovine genital campylobacteriosis (vibrio).
Note: If submitting a preputial washing for both Tritrichomonas and C. venerealis testing, samples cannot be pooled for either test as there is not enough processed specimen for individual testing if positive.
*Each additional test is $15.
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Species |
Bovine | Other
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Shipping Requirements |
Send on cold pack/ice in sterile leak-proof container within 24 hours. To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Additional Instructions |
Culture to confirm PCR positive results is recommended. For other Campylobacter species see Campylobacter species (PCR). Plastic tubes are preferred. If blood tubes are used, must be red top without serum separator gel or anticoagulant.
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay differentiates select campylobacter species from culture submissions by targeting DNA fragments of the ISCfe-1 gene from C. fetus subsp. venerealis, nahE gene from C. fetus, and the cj0414 oxidoreductase gene from C. jejuni.
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Species |
Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of canine coronavirus by fluorescent antibody (FA) testing.
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Species |
Canine
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of canine distemper virus by fluorescent antibody (FA) testing.
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Species |
Canine
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Acceptable Samples |
EDTA whole blood (1 ml minimum); Swabs (conjunctiva, oropharyngeal, rectal)
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This targets the RNA of a portion of the N protein gene from the Canine distemper virus. Please be aware that this assay could detect vaccine strains if vaccination occurs within a few days or weeks of testing. This assay is available as part of the Canine Respiratory Panel.
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Species |
Canine Wildlife Exotic Other
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Additional Instructions |
To ensure inclusion in the test run, samples should arrive the day prior to test day. Submit swabs in sterile, redtop tubes (without additives) or other sterile screwcap containers to with a small amount of saline (enough to cover the fibrous head of the swab) to maintain moisture. Viral transport media and universal transport media are also acceptable. Gel, such as culturette media or the type found in serum separator tubes can inhibit PCR and should not be tested. Bacterial transport containers are not appropriate for PCR testing. If bacterial culture is also requested, please submit a second swab in the appropriate bacterial transport container.
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Acceptable Samples |
Ocular swab
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the DNA of a portion of the glycoprotein B gene from Canine herpesvirus-1.
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Species |
Canine
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Additional Instructions |
To ensure inclusion in the test run, samples should arrive the day prior to test day. Submit swabs in sterile, redtop tubes (without additives) or other sterile screwcap containers to with a small amount of saline (enough to cover the fibrous head of the swab) to maintain moisture. Viral transport media and universal transport media are also acceptable. Gel, such as culturette media or the type found in serum separator tubes, can inhibit PCR and should not be tested. Bacterial transport containers are not appropriate for PCR testing. If bacterial culture is also requested, please submit a second swab in the appropriate bacterial transport container. CHV-1 PCR testing should be focused on neonatal puppies, where the virus can cause fatal hepatic and renal necrosis. Adult dogs are susceptible to CHV-1, but the infection is usually asymptomatic or subclinical. Due to the potential for reactivation of a latent infection, CHV-1 PCR should be used as a tool for confirmation of a clinical diagnosis.
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of canine parvovirus 2 by fluorescent antibody (FA) testing.
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Species |
Canine
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Additional Instructions |
Does not react with canine parvovirus type 1.
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Acceptable Samples |
Canine: Lung, liver, spleen, kidney, heart | Feline: Cerebellum
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the DNA of a portion of theVP2 gene from Canine parvovirus-2 and Feline panleukopenia virus. This assay cannot differentiate CPV-2 and FPLV.
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Species |
Canine | Feline
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Additional Instructions |
The virus is usually only detectable in the feces for up to 2 weeks after infection.
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Acceptable Samples |
Lithium heparin plasma (1 ml minimum)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time Same day if received by 1:00 p.m.
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Description |
Quantitative in vitro determination of canine progesterone by immunoassay.
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Species |
Canine
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Shipping Requirements |
Submit samples on ice.
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Acceptable Samples |
Lymph node, tonsil, trachea
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the RNA of a portion of the nucleocapsid gene from Canine respiratory coronavirus. This assay does not detect intestinal coronavirus. See Canine Coronavirus (FA). This assay can be multiplexed, see Canine Respiratory Panel.
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Species |
Canine
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Additional Instructions |
Submit swabs in sterile, redtop tubes (without additives) or other sterile screwcap containers to with a small amount of saline (enough to cover the fibrous head of the swab) to maintain moisture. Viral transport media and universal transport media are also acceptable. Gel, such as culturette media or the type found in serum separator tubes, can inhibit PCR and should not be tested. Bacterial transport containers are not appropriate for PCR testing. If bacterial culture is also requested, please submit a second swab in the appropriate bacterial transport container.
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Schedule |
Turnaround Time PCR 1-3 business days from test day; Culture 2-5 days
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Description |
This assay targets the RNA of a portion of the matrix gene of the Influenza A virus, N protein gene from the Canine distemper virus, and nucleocapsid gene from Canine respiratory coronavirus. Please be aware that this assay could detect vaccine strains if vaccination occurs within a few days or weeks of testing.
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Species |
Canine
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Additional Instructions |
Panel includes canine influenza A virus (all known types), canine distemper virus, canine respiratory coronavirus and aerobic culture for bacterial respiratory pathogens (Bordetella spp., Mycoplasma spp., Streptococcus spp., hemolytic E. coli, etc).
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Acceptable Samples |
Slides (charged/adhesive)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Identification of CD3 (T cell) markers by immunohistochemistry using anti-CD3 monoclonal antibody (clone LN10).
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Species |
Canine | Feline
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Shipping Requirements |
In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.
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Acceptable Samples |
Slides (charged/adhesive)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Identification of CD79a (B cell) markers by immunohistochemistry using anti-CD79a monoclonal antibody (clone HM57).
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Species |
Canine | Feline
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Shipping Requirements |
In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.
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Acceptable Samples |
Lithium heparin plasma (1 ml minimum)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time Same day results if received by 1:00 p.m.
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Description |
Automated wet chemistry analyzing albumin (ALB), alkaline phosphatase (ALKP), aspartate aminotransferase (AST), blood urea nitrogen (BUN), calcium (Ca), creatine kinase (CK), gamma-glutamyltransferase (GGT), globulin (Glob), phosphorus (P) magnesium (Mg), total protein (TP). Reference intervals not available for all species (non domestic).
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Species |
Equine | Bovine | Ovine | Caprine | Porcine | Other
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Shipping Requirements |
Spin down sample and aliquot serum/plasma to a separate tube. Send serum/plasma aliquot on ice.
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Acceptable Samples |
Lithium heparin (1 ml minimum)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time Same day results if received by 1:00 p.m.
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Description |
Automated measurement of albumin (ALB), alkaline phosphatase (ALKP), alanine aminotransferase (ALT), calcium (Ca), cholesterol (CHOL), creatinine (CREA), glucose (GLU), phosphorous (P), total bilirubin (TBIL), total protein (TP), and blood urea nitrogen (BUN). Reference intervals not available for all species.
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Species |
Canine | Feline | Rabbit | Mouse | Other
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Shipping Requirements |
Spin down sample and aliquot serum/plasma to a separate tube. Send serum/plasma aliquot on ice.
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Acceptable Samples |
Vaginal swab, Tissue (synovium, placenta, fetal organs)
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the DNA of a portion of the 23S rRNA gene from bacterial Chlamydiae species C. abortus (previously C. psittaci), C. pecorum (previously C. psittaci serotype 2), and C. psittaci.
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Species |
Ovine | Caprine | Wildlife Exotic | Other
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Additional Instructions |
Submit swabs in sterile, redtop tubes (without additives) or other sterile screwcap containers to with a small amount of saline (enough to cover the fibrous head of the swab) to maintain moisture. Viral transport media and universal transport media are also acceptable. Gel, such as culturette media or the type found in serum separator tubes, can inhibit PCR and should not be tested. Bacterial transport containers are not appropriate for PCR testing. If bacterial culture is also requested, please submit a second swab in the appropriate bacterial transport container.
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Acceptable Samples |
Tonsil scraping, swab
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This is a NAHLN assay; approval to conduct surveillance testing is based upon satisfactory completion of the yearly proficiency test.
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Species |
Porcine
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Shipping Requirements |
Samples must be received by 11:00 am of the test day to ensure the reported turn around time.
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of Clostridium chauvoei by fluorescent antibody (FA) testing.
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Acceptable Samples |
EDTA whole blood (1 ml minimum)
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the DNA of a portion of the flagellin (fliC) gene from the bacterium Clostridium haemolyticum.
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Species |
Bovine | Equine | Ovine | Porcine | Wildlife Exotic | Other
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Additional Instructions |
Antibiotic treatment may interfere with PCR detection.
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of Clostridium novyi by fluorescent antibody (FA) testing.
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Schedule |
Turnaround Time 1-3 business days from test day
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Description |
This assay targets the DNA of the toxin genes cpa (α-toxin), cpb (β-toxin), etx (ε-toxin), itxA (ι-toxin), and cpe (enterotoxin) from the bacterium Clostridium perfringens.
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Species |
Bovine | Canine | Equine | Ovine | Porcine
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Shipping Requirements |
To ensure inclusion in the test run, samples should arrive the day prior to test day.
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Acceptable Samples |
Swabs
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Isolation and identification of Clostridium perfringens from diagnostic samples.
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Species |
Bovine | Canine | Feline | Equine | Caprine | Porcine | Wildlife Exotic | Other
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Shipping Requirements |
Send samples on ice pack in a sterile, leak-proof container. Overnight or 2 day shipping for samples is recommended.
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of Clostridium septicum by fluorescent antibody (FA) testing.
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time 24-48 hours
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Description |
Detection of Clostridium sordellii by fluorescent antibody (FA) testing.
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Acceptable Samples |
EDTA whole blood (1 ml minimum)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time Same day results if received by 1:00 p.m.
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Description |
Automated complete blood count with 3-part differential (neutrophils, lymphocytes, and monocytes) and flagging of eosinophils. Additionally, a manual differential with WBC count, estimate and morphologic changes (if any), RBC morphologic changes (if any), platelet estimate and morphologic changes (if any), packed cell volume, and total protein (if serum was also submitted. Reference ranges provided for common domestic species only.
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Species |
Bovine | Canine | Feline | Equine | Ovine | Porcine
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Shipping Requirements |
Blood films should be prepared immediately after collection and air dried. No need to stain. If tests are not performed within 2 hours of collection, they should be refrigerated. Samples should be shipped on ice.
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Schedule |
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Description |
Quantitation of copper by inductively coupled plasma atomic emission spectroscopy (ICP-OES).
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Shipping Requirements |
Samples must be received 2:00 pm Wednesday for inclusion in the Thursday test run.
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Acceptable Samples |
Slides (charged/adhesive)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Dectection of Cryptosporidium species by modified acid-fast stain.
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Species |
Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Determination of cyanide potential by ion-selective electrode (ISE).
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Species |
Feed Forage
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Shipping Requirements |
Seal in plastic bag and ship in cardboard box. Do NOT send frozen. Samples chopped, frosted, frozen or crimped may give off cyanide for several days, and analysis may not reflect accurate cyanide potential. Overnight or next-day delivery of samples preferred.
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Acceptable Samples |
Slides (charged/adhesive)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
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Description |
Immunohistochemistry using multi-cytokeratin (AE1/AE3) polyclonal antibodies.
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Species |
Canine | Feline
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Shipping Requirements |
In cold weather, add 1 part ethanol to 9 parts 10% buffered formalin to prevent freezing during transit.
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Acceptable Samples |
Fluid in EDTA tube and sterile serum tube (RTT), (0.5 ml minimum)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time Same day results if received by 1:00 p.m.
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Description |
Pathologist evaluation of cytologic samples. Cytologic samples may be collected by swabbing, scraping, and/or aspirating the lesion. Cytologic smears should be prepared immediately after collection and air dried.
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Species |
Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine
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Shipping Requirements |
Ship samples on ice. Submit slides in a protective slide case holder. Never submit a syringe with a needle attached. Swabs in gel transport media are not acceptable for cytology. If submitting a swab in a sterile tube add a few drops of sterile saline so it does not become dry while being transported to lab.
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Additional Instructions |
Slides from each additional site are $6.00.
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Acceptable Samples |
Fluid in RTT only (0.5 ml minimum)
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Schedule |
Days Tested MON | TUE | WED | THU | FRI
Turnaround Time Same day results if received by 1:00 p.m.
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Description |
Pathologist evaluation of fluid samples submitted for cytology. Includes gross description of the fluid and a total protein.
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Species |
Bovine | Canine | Feline | Equine | Ovine | Caprine | Porcine | Wildlife Exotic | Other
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Shipping Requirements |
Ship samples on ice. Never submit a syringe with a needle attached.
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