Service Announcement
August 26, 2009
BVD PCR
EAR NOTCH TESTING
The NDSU-VDL frequently evaluates BVDV detection strategies. Immunohistochemical
analysis of formalin-fixed samples has been the method offered for detecting PI
animals. Based on the validation of a newer method, the most cost effective and
sensitive strategy we can now offer is to test pools of 15 ear notches by PCR, and
then individually test samples within positive pools. The cost per pool is $30.00
for the first pool, and $15.00 for each additional pool. Samples will be run twice
a week, usually on Tuesday and Thursday. Please contact the laboratory if you are
planning on sending in a large number of notches. Individuals from positive tubes
will be retested using either PCR or antigen capture ELISA testing after discussion
with the referring veterinarian and owner. It is recommended that positive individuals
be retested again a month later to confirm their PI status.
Immunohistochemical analysis
of formalin-fixed tissues will continue to be available for testing smaller numbers
of notches. The price is $20.00 for one sample and $25.00 for up to six samples
on a slide.
Interpreting results:
- “BVDV Not Detected”
- “Positive” - means
the virus was identified in the pool - may be the result of a persistently infected
animal or an acutely infected animal.
Equipment Needed:
- Ear Notching tool that
yields a 1cm square notch
- Red Top blood tube (no
additive or gel). Formalin is NOT
needed.
This assay uses
reagents that are provided by the lab for PCR analysis.
- Shipping container that
holds individual consecutively labeled tubes in slots.
- Disinfectant for rinsing
the notching tool; 10% bleach will work.
- Clean rinse water (between
notching and disinfection). 6. Do not vaccinate or tattoo at the same time samples
are taken.
Collection Procedure:
- Label red top collection tubes with the animal IDs and number the tubes consecutively.
- Dip notching tool in disinfectant then rinse away disinfectant with clean water.
Residual disinfectant on the notching tool will give false negative results, therefore
thorough rinsing is required.
- Take a proper size ear notch (approximately 1 cm X 1 cm) from a clean portion
of the ear. Place notch into a labeled, dry, red top tube.
- Record animal identification numbers on a serology request form.
Ear notch must be free of dirt, feces, tattoo ink or BVD vaccine.
How to ship:
Ship on ice. If the sample will not get to the lab the next day, freeze the sample
and ship on ice. Samples may be kept in the freezer up to one month and shipped
together. The shipping address is:
Post Office Mail Address:
Veterinary Diagnostic Laboratory
NDSU Dept. 7691
PO Box 6050
Fargo, ND 58108-6050
UPS or Fed Ex Address:
Veterinary Diagnostic Laboratory
NDSU Van Es Hall
1523 Centennial Blvd.
Fargo, ND 58102
See original message
Please contact the laboratory if you have any questions (701-231-8307).